Review



kb mil 18 dna expression plasmid vector  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Thermo Fisher kb mil 18 dna expression plasmid vector
    Detection of <t>mIL-18</t> plasmid expression in DNA treatment sites . BALB/c mice were inoculated with murine CT26 cells in the left liver lobe, and treated intratumorally with varying doses of control plasmid or that expressing IL-18 until day 7 after tumor inoculation. Liver tissue was obtained on day 4 after direct injection of IL-18 plasmid DNA, and ELISA used to evaluate IL-18 protein levels. Representative data determined from two separate experiments.
    Kb Mil 18 Dna Expression Plasmid Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+mil+18+dna+expression+plasmid+vector/Deoxyribonucleic+acid/pmc01903361-32-2-19
    Average 99 stars, based on 1 article reviews
    kb mil 18 dna expression plasmid vector - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model"

    Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-7-87

    Detection of mIL-18 plasmid expression in DNA treatment sites . BALB/c mice were inoculated with murine CT26 cells in the left liver lobe, and treated intratumorally with varying doses of control plasmid or that expressing IL-18 until day 7 after tumor inoculation. Liver tissue was obtained on day 4 after direct injection of IL-18 plasmid DNA, and ELISA used to evaluate IL-18 protein levels. Representative data determined from two separate experiments.
    Figure Legend Snippet: Detection of mIL-18 plasmid expression in DNA treatment sites . BALB/c mice were inoculated with murine CT26 cells in the left liver lobe, and treated intratumorally with varying doses of control plasmid or that expressing IL-18 until day 7 after tumor inoculation. Liver tissue was obtained on day 4 after direct injection of IL-18 plasmid DNA, and ELISA used to evaluate IL-18 protein levels. Representative data determined from two separate experiments.

    Techniques Used: Plasmid Preparation, Expressing, Injection, Enzyme-linked Immunosorbent Assay

    Immune response is enhanced by a single injection of naked IL-18 plasmid DNA . Each group (consisting of 4 mice) was administered an intratumoral injection of pCEP4 or naked mIL-18 DNA. Spleens of CT26 tumor-bearing mice treated with plasmids were harvested at 7 days after plasmid injection, and cells analyzed by flow cytometry using the corresponding FITC- or PE- or PE-Cy5-labeled antibodies and isotype control.
    Figure Legend Snippet: Immune response is enhanced by a single injection of naked IL-18 plasmid DNA . Each group (consisting of 4 mice) was administered an intratumoral injection of pCEP4 or naked mIL-18 DNA. Spleens of CT26 tumor-bearing mice treated with plasmids were harvested at 7 days after plasmid injection, and cells analyzed by flow cytometry using the corresponding FITC- or PE- or PE-Cy5-labeled antibodies and isotype control.

    Techniques Used: Injection, Plasmid Preparation, Flow Cytometry, Labeling

    IL-18 gene transfer results in regression of CT26 liver tumors . CT26 cells (1.0 × 10 5 ) were injected into a left lobe of the livers of 6-8-week-old BALB/c mice. pCEP4 or mIL-18 plasmid DNA was injected directly into the tumors on day 7. Tumor growth was measured on days 1, 4, 7, and 14 after plasmid implantation. Data are presented as the mean of tumor weights of 8 mice/group.
    Figure Legend Snippet: IL-18 gene transfer results in regression of CT26 liver tumors . CT26 cells (1.0 × 10 5 ) were injected into a left lobe of the livers of 6-8-week-old BALB/c mice. pCEP4 or mIL-18 plasmid DNA was injected directly into the tumors on day 7. Tumor growth was measured on days 1, 4, 7, and 14 after plasmid implantation. Data are presented as the mean of tumor weights of 8 mice/group.

    Techniques Used: Injection, Plasmid Preparation

    Intracellular IFN-γ production by CT26-specific T cells from pmIL-18-treated mice . Splenocytes from CT26-tumor bearing mice treated with three rounds of pCEP4 or mIL-18 were pooled and incubated in medium alone or that containing CT26 lysates. Numbers within the gates in the FACS plots depict the percentage of CD69 + IFN-γ + CD4 + cells. Data are presented from experiments that were repeated at least twice.
    Figure Legend Snippet: Intracellular IFN-γ production by CT26-specific T cells from pmIL-18-treated mice . Splenocytes from CT26-tumor bearing mice treated with three rounds of pCEP4 or mIL-18 were pooled and incubated in medium alone or that containing CT26 lysates. Numbers within the gates in the FACS plots depict the percentage of CD69 + IFN-γ + CD4 + cells. Data are presented from experiments that were repeated at least twice.

    Techniques Used: Incubation

    Elevated IFN-γ levels after intratumoral injection of nonviral mIL-18 plasmid DNA . Splenocytes from CT26 tumor-bearing mice after gene therapy were collected at 4, 7, and 14 days after gene treatment, and cultured with anti-CD3 and anti-CD28 antibodies for 3 days before performing ELISA (* = 0.006).
    Figure Legend Snippet: Elevated IFN-γ levels after intratumoral injection of nonviral mIL-18 plasmid DNA . Splenocytes from CT26 tumor-bearing mice after gene therapy were collected at 4, 7, and 14 days after gene treatment, and cultured with anti-CD3 and anti-CD28 antibodies for 3 days before performing ELISA (* = 0.006).

    Techniques Used: Injection, Plasmid Preparation, Cell Culture, Enzyme-linked Immunosorbent Assay

    Related Articles

    Expressing:

    Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model
    Article Snippet: CT26 tumor cells were maintained in RPMI 1640 cell culture medium (Biowhittaker, Walkersville, MD, USA) supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 100 U/ml streptomycin, and 100 μg/ml penicillin. .. The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene. .. Plasmid DNA was purified in the absence of ethidium bromide using a commercial column chromatography method, according to the manufacturer's protocol (Qiagen, Chatsworth, CA).

    Plasmid Preparation:

    Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model
    Article Snippet: CT26 tumor cells were maintained in RPMI 1640 cell culture medium (Biowhittaker, Walkersville, MD, USA) supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 100 U/ml streptomycin, and 100 μg/ml penicillin. .. The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene. .. Plasmid DNA was purified in the absence of ethidium bromide using a commercial column chromatography method, according to the manufacturer's protocol (Qiagen, Chatsworth, CA).

    Construct:

    Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model
    Article Snippet: CT26 tumor cells were maintained in RPMI 1640 cell culture medium (Biowhittaker, Walkersville, MD, USA) supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 100 U/ml streptomycin, and 100 μg/ml penicillin. .. The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene. .. Plasmid DNA was purified in the absence of ethidium bromide using a commercial column chromatography method, according to the manufacturer's protocol (Qiagen, Chatsworth, CA).

    Selection:

    Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model
    Article Snippet: CT26 tumor cells were maintained in RPMI 1640 cell culture medium (Biowhittaker, Walkersville, MD, USA) supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 100 U/ml streptomycin, and 100 μg/ml penicillin. .. The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene. .. Plasmid DNA was purified in the absence of ethidium bromide using a commercial column chromatography method, according to the manufacturer's protocol (Qiagen, Chatsworth, CA).



    Similar Products

    kb mil 18 dna expression plasmid vector  (Thermo Fisher)
    99
    Thermo Fisher kb mil 18 dna expression plasmid vector
    Detection of <t>mIL-18</t> plasmid expression in DNA treatment sites . BALB/c mice were inoculated with murine CT26 cells in the left liver lobe, and treated intratumorally with varying doses of control plasmid or that expressing IL-18 until day 7 after tumor inoculation. Liver tissue was obtained on day 4 after direct injection of IL-18 plasmid DNA, and ELISA used to evaluate IL-18 protein levels. Representative data determined from two separate experiments.
    Kb Mil 18 Dna Expression Plasmid Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+mil+18+dna+expression+plasmid+vector/Deoxyribonucleic+acid/pmc01903361-32-2-19
    Average 99 stars, based on 1 article reviews
    kb mil 18 dna expression plasmid vector - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Detection of mIL-18 plasmid expression in DNA treatment sites . BALB/c mice were inoculated with murine CT26 cells in the left liver lobe, and treated intratumorally with varying doses of control plasmid or that expressing IL-18 until day 7 after tumor inoculation. Liver tissue was obtained on day 4 after direct injection of IL-18 plasmid DNA, and ELISA used to evaluate IL-18 protein levels. Representative data determined from two separate experiments.

    Journal: BMC Cancer

    Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model

    doi: 10.1186/1471-2407-7-87

    Figure Lengend Snippet: Detection of mIL-18 plasmid expression in DNA treatment sites . BALB/c mice were inoculated with murine CT26 cells in the left liver lobe, and treated intratumorally with varying doses of control plasmid or that expressing IL-18 until day 7 after tumor inoculation. Liver tissue was obtained on day 4 after direct injection of IL-18 plasmid DNA, and ELISA used to evaluate IL-18 protein levels. Representative data determined from two separate experiments.

    Article Snippet: The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene.

    Techniques: Plasmid Preparation, Expressing, Injection, Enzyme-linked Immunosorbent Assay

    Immune response is enhanced by a single injection of naked IL-18 plasmid DNA . Each group (consisting of 4 mice) was administered an intratumoral injection of pCEP4 or naked mIL-18 DNA. Spleens of CT26 tumor-bearing mice treated with plasmids were harvested at 7 days after plasmid injection, and cells analyzed by flow cytometry using the corresponding FITC- or PE- or PE-Cy5-labeled antibodies and isotype control.

    Journal: BMC Cancer

    Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model

    doi: 10.1186/1471-2407-7-87

    Figure Lengend Snippet: Immune response is enhanced by a single injection of naked IL-18 plasmid DNA . Each group (consisting of 4 mice) was administered an intratumoral injection of pCEP4 or naked mIL-18 DNA. Spleens of CT26 tumor-bearing mice treated with plasmids were harvested at 7 days after plasmid injection, and cells analyzed by flow cytometry using the corresponding FITC- or PE- or PE-Cy5-labeled antibodies and isotype control.

    Article Snippet: The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene.

    Techniques: Injection, Plasmid Preparation, Flow Cytometry, Labeling

    IL-18 gene transfer results in regression of CT26 liver tumors . CT26 cells (1.0 × 10 5 ) were injected into a left lobe of the livers of 6-8-week-old BALB/c mice. pCEP4 or mIL-18 plasmid DNA was injected directly into the tumors on day 7. Tumor growth was measured on days 1, 4, 7, and 14 after plasmid implantation. Data are presented as the mean of tumor weights of 8 mice/group.

    Journal: BMC Cancer

    Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model

    doi: 10.1186/1471-2407-7-87

    Figure Lengend Snippet: IL-18 gene transfer results in regression of CT26 liver tumors . CT26 cells (1.0 × 10 5 ) were injected into a left lobe of the livers of 6-8-week-old BALB/c mice. pCEP4 or mIL-18 plasmid DNA was injected directly into the tumors on day 7. Tumor growth was measured on days 1, 4, 7, and 14 after plasmid implantation. Data are presented as the mean of tumor weights of 8 mice/group.

    Article Snippet: The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene.

    Techniques: Injection, Plasmid Preparation

    Intracellular IFN-γ production by CT26-specific T cells from pmIL-18-treated mice . Splenocytes from CT26-tumor bearing mice treated with three rounds of pCEP4 or mIL-18 were pooled and incubated in medium alone or that containing CT26 lysates. Numbers within the gates in the FACS plots depict the percentage of CD69 + IFN-γ + CD4 + cells. Data are presented from experiments that were repeated at least twice.

    Journal: BMC Cancer

    Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model

    doi: 10.1186/1471-2407-7-87

    Figure Lengend Snippet: Intracellular IFN-γ production by CT26-specific T cells from pmIL-18-treated mice . Splenocytes from CT26-tumor bearing mice treated with three rounds of pCEP4 or mIL-18 were pooled and incubated in medium alone or that containing CT26 lysates. Numbers within the gates in the FACS plots depict the percentage of CD69 + IFN-γ + CD4 + cells. Data are presented from experiments that were repeated at least twice.

    Article Snippet: The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene.

    Techniques: Incubation

    Elevated IFN-γ levels after intratumoral injection of nonviral mIL-18 plasmid DNA . Splenocytes from CT26 tumor-bearing mice after gene therapy were collected at 4, 7, and 14 days after gene treatment, and cultured with anti-CD3 and anti-CD28 antibodies for 3 days before performing ELISA (* = 0.006).

    Journal: BMC Cancer

    Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model

    doi: 10.1186/1471-2407-7-87

    Figure Lengend Snippet: Elevated IFN-γ levels after intratumoral injection of nonviral mIL-18 plasmid DNA . Splenocytes from CT26 tumor-bearing mice after gene therapy were collected at 4, 7, and 14 days after gene treatment, and cultured with anti-CD3 and anti-CD28 antibodies for 3 days before performing ELISA (* = 0.006).

    Article Snippet: The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene.

    Techniques: Injection, Plasmid Preparation, Cell Culture, Enzyme-linked Immunosorbent Assay